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제목 Biolegend 한국대리점 - Phosphor Antibody출시
글쓴이 학술부 작성일 2018-02-27 16:22:26

Phospho Antibody 출시


AntibodyApplications
Alexa Fluor® 647 Anti-ERK1/2 Phospho (Thr 202/Tyr204) (Clone 4B11B69)ICFC
Purified Anti-ERK1/2 Phospho (Thr 202/Tyr204) (Clone 4B11B69)WBIF


Alexa Fluor® 647 anti-ERK1/2 Phospho (Thr202/Tyr204) Antibody (clone 4B11B69)
Intracellular Flow Cytometry (ICFC):
Human peripheral blood lymphocytes were stimulated with (filled histogram), or without (open histogram), Cell Activation Cocktail (without Brefeldin A) for 15 minutes, then fixed with Fixation Buffer, permeabilized with True-Phos™ Perm Buffer, and intracellularly stained with ERK1/2 Phospho (Thr202/Tyr204) (Clone 4B11B69) Alexa Fluor® 647.C57BL/6 mouse splenocytes were stimulated with (filled histogram) or without (open histogram) Cell Activation Cocktail (without Brefeldin A) for 15 minutes, then fixed with Fixation Buffer, permeabilized with True-Phos™ Perm Buffer, and intracellularly stained with ERK1/2 Phospho (Thr202/Tyr204) (Clone 4B11B69) Alexa Fluor® 647.

Products Referenced Above:

Product NameCatalog #
Alexa Fluor® 647 anti-ERK1/2 Phospho (Thr202/Tyr204) Antibody675503 | 675504
Cell Activation Cocktail (without Brefeldin A)423301 | 423302
Fixation Buffer420801
True-Phos™ Perm Buffer425401

Purified anti-ERK1/2 Phospho (Thr202/Tyr204) Antibody (clone 4B11B69)
Western Blot (WB):
Left: Untreated (lane 1) and PMA-treated (lane 2) Jurkat whole cell lysates were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-ERK1/2 Phospho (Thr202/Tyr204) (clone 4B11B69) antibody. Proteins were visualized using an anti-mouse-IgG secondary conjugated to HRP (Cat. No. 405306) and chemiluminescence detection.
Immunofluorescence (IF):
Above: Untreated (left) and PMA-treated (20 nM PMA treatment for 10 minutes, right) mouse NIH-3T3 cells were stained with purified anti-ERK1/2 Phospho (Thr202/Tyr204) (clone 4B11B69) antibody, followed by staining with DyLight™ 488 conjugated goat anti-mouse IgG (green, Cat. No. 405310) antibody and Alexa Fluor® 594 conjugated phalloidin (red). Nuclei were stained with DAPI (blue).

Products Referenced Above:

Product NameCatalog #
Purified anti-ERK1/2 Phospho (Thr202/Tyr204) Antibody675502
HRP Goat anti-mouse IgG (minimal x-reactivity) Antibody (WB)405306
DyLight™ 488 Goat anti-mouse IgG (minimal x-reactivity) Antibody (IF)405310
DAPI (4', 6-Diamidino-2-Phenylindole, Dilactate) (IF)422801


SpecificityClone
BTK Phospho (Tyr223)
A16128B
BTK Phospho (Tyr223)/ITK Phospho (Tyr180) A16128C
BTK Phospho (Tyr551)/ITK Phospho (Tyr512)
A16064A
ERK1/2 Phospho (Thr202/Tyr204) 6B8B69
p38 MAPK Phospho (Thr180/Tyr182)
Poly29035
STAT3 Phospho (Tyr705) 13A3-1
STAT3 Phospho (Ser727)
A16089B
STAT6 Phospho (Tyr641) A15137A



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