G-418 Sulfate

G-418 Sulfate (also known commercially as Geneticin) is an aminoglycoside antibiotic that is synthesized from Micromonospora rhodorangea and functions by causing a chain of nonsense mutations during translation. Due to translation G-418 inhibited polypeptide synthesis and protein elongation. G-418 binds to the 80S subunit of the ribosome by blocking the elongation phase of protein synthesis, both in prokaryotic and eukaryotic cells. It also shows activity against protozoa, cecal amoebiasis, and helminths.

Chemical Data

Chemical Formula:	C20H40O10N4 • 2H2SO4
CAS Number:	108321-42-2
Molecular Weight:	692.7 Da
Appearance:	White to off-white powder
Purity:	≥92%

Applications

• Used routinely in molecular genetics experiments for selecting eukaryotic transformants
• Used in plant and mammalian cell culture as a selectable marker
• Used as a gene selection antibiotic that has a powerful new role in genome editing, utilizing the CRISPR/CAS9 system.
• Ability to inhibit poliovirus and rhinovirus 2A proteinases in vitro