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TotalSeq™ : Single-Cell Proteogenomics

TotalSeq™ oligo-conjugated antibodies enable measurement of proteins at a single cell level and integrate seamlessly into existing single-cell RNA sequencing workflows, including Drop-Seq and those available from 10x Genomics.

 

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TotalSeq™-C Human TBNK Cocktail

Cat # 399903

TotalSeqC_TBNK_Panel_Figure_022420

Major immune cell lineages are identified using the TotalSeq™-C TBNK Cocktail. Human peripheral blood mononuclear cells were stained with the TotalSeq™-C TBNK Cocktail and processed using the 10x Genomics Single Cell 5' v1.1 feature barcoding kit and Illumina sequencing. Protein count data were transformed and visualized in a UMAP projection overlayed with protein expression levels for each component of the cocktail. Cell clusters were identified based on protein expression only.

Barcode ID Specificity Clone Barcode sequence
C0050 CD19 HIB19 CTGGGCAATTACTCG
C0034 CD3 UCHT1 CTCATTGTAACTCCT
C0083 CD16 3G8 AAGTTCACTCTTTGC
C0072 CD4 RPA-T4 TGTTCCCGCTCAACT
C0053 CD11c S-HCL-3 TACGCCTATAACTTG
C0047 CD56 (NCAM) 5.1H11 TCCTTTCCTGATAGG
C0081 CD14 M5E2 TCTCAGACCTCCGTA
C0046 CD8 SK1 GCGCAACTTGATGAT
C0048 CD45 2D1 TCCCTTGCGATTTAC