
TotalSeq™ : Single-Cell Proteogenomics

TotalSeq™ oligo-conjugated antibodies enable measurement of proteins at a single cell level and integrate seamlessly into existing single-cell RNA sequencing workflows, including Drop-Seq and those available from 10x Genomics.

Cat # 399903

Major immune cell lineages are identified using the TotalSeq™-C TBNK Cocktail. Human peripheral blood mononuclear cells were stained with the TotalSeq™-C TBNK Cocktail and processed using the 10x Genomics Single Cell 5' v1.1 feature barcoding kit and Illumina sequencing. Protein count data were transformed and visualized in a UMAP projection overlayed with protein expression levels for each component of the cocktail. Cell clusters were identified based on protein expression only.
Barcode ID |
Specificity |
Clone |
Barcode sequence |
C0050 |
CD19 |
HIB19 |
CTGGGCAATTACTCG |
C0034 |
CD3 |
UCHT1 |
CTCATTGTAACTCCT |
C0083 |
CD16 |
3G8 |
AAGTTCACTCTTTGC |
C0072 |
CD4 |
RPA-T4 |
TGTTCCCGCTCAACT |
C0053 |
CD11c |
S-HCL-3 |
TACGCCTATAACTTG |
C0047 |
CD56 (NCAM) |
5.1H11 |
TCCTTTCCTGATAGG |
C0081 |
CD14 |
M5E2 |
TCTCAGACCTCCGTA |
C0046 |
CD8 |
SK1 |
GCGCAACTTGATGAT |
C0048 |
CD45 |
2D1 |
TCCCTTGCGATTTAC |
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