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Spectral cytometry allows for the simultaneous use of fluorophores with spectral characteristics too similar to be distinguished from each other on conventional cytometers. PE/Fire™ 640 has a distinct emission peak between the peaks of PE/Dazzle™ 594 and PE/Cyanine 5, which allows it to be accurately unmixed from these highly overlapping fluorophores. PE/Fire™ 640 was designed to be used for spectral cytometry, but may be used on conventional cytometers with filters optimized by the end user. PE/Fire™ 640 is recommended for: (1) labeling an antigen that is expressed on a small subset of cells in the sample, (2) labeling an antigen that will be excluded from final analysis, and (3) labeling an antigen that requires a bright signal but is not co-expressed with an antigen detected by PE/Cyanine5.

PE/Fire™ 640

	Brightness: 4
	Ex Laser: 561 nm
	Ex Max: 620 nm
	Em Max: 639 nm
	Ideal for use in spectral cytometry. For conventional cytometry, it may need to be tested/optimized for filters by the end user.
	Molecular Weight: 260 kDa
	Recommended Application(s): Flow Cytometry
	Typical F:P Ratio: 1 to 1 *This is a general fluorophore to protein ratio range. For a lot-specific value, you can contact technical services.

Fluorophore At a Glance:

• Bright signal with little background.
• Recommended for staining an antigen that is expressed on a small subset of cells.
• Can be used in the same panel as PE/Cyanine5 but ideally, they should be used to detect two markers that are not co-expressed.
• Comparable compensation requirements into PE when compared to spectrally equivalent tandems.
• Comparable temperature and photostability when compared to spectrally equivalent tandems.
• PE/Fire™ 640 may exhibit non-specific binding to monocytes and macrophages. Use our True-Stain Monocyte Blocker™ before staining to block this non-specific binding.

View all PE/Fire™ 640 Products.
 

Learn more about PE/Fire™ 640.

Multicolor Compatibility of PE/Fire™ 640

RBC lysed and washed human blood was stained with optimal test concentrations of the indicated antibodies. Staining was performed in the presence of appropriate blocking buffers, including True-Stain Monocyte Blocker™.

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