Understand how SARS-CoV-2 recognizes and invades cells with our newly released anti-human ACE2 antibody. It has been validated for use in both immunohistochemistry and western blotting applications.
Purified anti-human ACE2 Antibody
Cat # 375801, 375802
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Human paraffin-embedded kidney tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Sodium Citrate H.I.E.R. 1X at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween 20 twice for five minutes, permeabilized with 0.5% Triton X-100 for ten minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of purified anti-human ACE2 (clone A20069I), and Nestin (clone 10C2) Alexa Fluor® 594 (green) at 4°C overnight, followed by 2.5 µg/mL of anti-rat IgG (clone Poly4054) Alexa Fluor® 647 (red) for two hours at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured with a 10X objective. |
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Human paraffin-embedded spleen tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Sodium Citrate H.I.E.R. 1X at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween 20 twice for five minutes, and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of purified anti-human ACE2 (clone A20069I), and anti-human CD45RA (clone HI100) Alexa Fluor® 594 (green) at 4°C overnight, followed by 2.5 µg/mL of anti-rat IgG (clone Poly4054) Alexa Fluor® 647 (red) for two hours at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured with a 10X objective. |
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Whole cell extracts (15 µg protein) from HeLa cells (negative control), human kidney and human testis tissue (positive controls), and mouse kidney tissue were resolved on a 4-12% Bis-Tris gel and transferred to a PVDF membrane. Membranes were probed with 0.1 µg/mL (1:5000 dilution) of purified anti-human ACE2 antibody (clone A20069I) for 2 hours at room temperature. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-actin antibody (Cat. No. 664804) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular weight marker.
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