ELISpot MAX™ Deluxe Set Human IFN-γ

Regulatory Status
Other Names
Interferon gamma, IFN-gamma
Cat no
5 plates

Human peripheral blood mononuclear cells (PBMC) at 104 cells /well were incubated for 20 hours in the absence or presence of 1X Cell Activator without Brefeldin A (Cat. No. 423301), 1X Cell Activator with Brefeldin A (Cat. No. 423303), or Ultra-LEAF™ purified anti-human CD3 (Cat. No. 300331). BioLegend’s Cell Activator cocktail contains PMA and ionomycin at the optimized concentration. Brefeldin A functions to inhibit intracellular protein transport. As a result, it inhibits secretion of cytokines such as IFN-γ. The number of cells secreting human IFN-γ was analyzed by ELISpot.
Kit Contents