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Our large columns are optimized for use with MojoSort on Columns™ reagents. Our columns can obtain greater purity at a lower cost and display equivalent flow rates to competitor's equivalent columns. For more information, explore our column data below.

MojoSort on Columns™ Cell Separation System is BioLegend’s new portfolio of reagents optimized for column-based magnetic cell separation. Our cell separation columns contain an iron sphere matrix, which generate a strong magnetic field when placed on BioLegend’s Column, Separator, and Multistand. Our column system can be used for positive or negative selection of MojoSort on Columns™ kits and reagents. In negative selection, the untouched cells flow through the column and are collected directly into a tube. In positive selection, the labeled cells are retained in the column matrix and eluted by removing the column from the magnetic separator.

MojoSort on Columns™ reagents and kits are optimized for use on our columns, separators, and stands. They have also demonstrated compatibility with third party columns, column separators and stands. Please contact BioLegend Technical Service for more guidance on compatibility with third party systems.

MojoSort on Columns™ - Human CD4 T Cell Isolation Kit

Untouched CD4+ T cells were isolated from fresh human whole blood PBMCs using the MojoSort on Columns™ - Human CD4 T Cell Isolation Kit with BioLegend’s Large Columns (Cat. No. 480178). The negatively selected cells were then stained with PE anti-human CD3 (clone UCHT1) (Cat. No. 300407) and APC anti-human CD4 (clone RPA-T4). The cells were then analyzed by flow cytometry gated on live CD45+ cells

MojoSort on Columns™ is BioLegend’s product line for column-based cell separation. This portfolio offers total solutions for column system setup as well as column-optimized formulations for reagents and kits.

This kit is designed for negative isolation of human CD4+ T cells from PBMCs harvested from whole blood and leukopaks. Non CD4+ cells are magnetically labeled by incubating your sample with the biotin antibody cocktail followed by magnetic Streptavidin Nanobeads. Once applied to our MojoSort on Columns™ System, the magnetically labeled cells are retained in the column matrix, while the untouched CD4+ T cells flow through the column and are collected into a clean tube. The depleted/labeled non CD4+ cells can be eluted from the column matrix for use as a control sample.

Below, we show an example workflow for MojoSort on Columns™ Human CD3 T Cell Isolation Kit and Human CD4 T Cell Isolation Kit. For more information on kit-specific protocols please visit our protocols section.

Graphical overview of the MojoSort on Columns protocol.

MojoSort™ on Columns

MojoSort on Columns™ - Human CD4 T Cell Isolation Kit

Protocol Overview