- Phosphate Buffered Saline (PBS): 80.0g NaCl
14.4g Na2HPO4, 2.4g KH2PO4, 2.0g KCl
Add ddH2O up to 10L; pH to 7.2 with HCl
- Coating Buffer: Can use either Sterile PBS or
Sterile Carbonate Buffer (per ELISA protocol)
8.4g Na HCO3, 3.56g Na2CO3, add ddH2O up to 1.0L, pH to 9.5.
- PBS-Tween:
0.05% Tween-20 in PBA (500µl Tween-20 in 1L PBS)
- Blocking Buffer (PBS-BSA):
1% BSA in PBS
(10 g BSA-Fraction V in 1L PBS)
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- PBS-Tween-BSA:
1% BSA in PBS-Tween
(10 g BSA-Fraction V in 1L PBS-Tween)
- Tissue Culture (TC) Medium:
As appropriate for cells being analyzed
- AEC Solution:
100 mg AEC (3-amino-9-ethyl-carbazole) in 10 ml DMF (N,N, Dimethylformamide)
Solution should be prepared in a glass tube in a fume hood.
- Substrate Solution:
800µl AEC solution in 24ml AEC buffer
Filter with 0.45µm filter and add 12µl 30% H2O2. Use immediately.
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