- 1X Cell Lysis Buffer: 20 mM Tris-HCl (pH 7.5), 150mM NaCl, 1% NP-40, 2 mM EDTA, 1µg/ml leupeptin, 1µg/ml aprotinin, 1 mM Na3PO4, 1 mM PMSF, 5 mM NaF, 3 mM Na4P2O4.
- 5X SDS Sample Buffer: 312.5 mM Tris-HCl (pH 6.8), 10% SDS (w/v), 250 mM DTT, 50% Glycerol, 0.05% Bromophenol Blue (w/v), Use at 1X.
- 10X SDS Running Buffer: Dissolve 144g of Glycine, 30g of Tris base and 10g SDS in 800ml of distilled H2O. Add distilled H2O to 1 liter. Use at 1X.
- Transfer Buffer: 3.0g Tris base, 14.4g Glycine, 200ml Methanol. Add deionized water to 1.0L.
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- Blocking Buffer: 1X TBS-T with 5% nonfat dry milk.
- Wash Buffer: 1X TBS-T.
- Primary and Secondary Antibody Dilution Buffer: 1X TBS-T with 5% nonfat dry milk.
- Alternate Blocking Buffer: 1X TBS-T with 4% Bovine Serum Albumin (BSA).
- Alternate Primary and Secondary Antibody Dilution Buffer: 1X TBS-T with 4% Bovine Serum Albumin (BSA).
- Blotting Membrane: Nitrocellulose or PVDF membrane.
- 10X TBS-T (Tris-buffered saline containing Tween-20): Dissolve 80g of NaCl, 2g of KCl, 30g of Tris base and 10ml Tween-20 in 800ml of distilled H2O. Adjust the pH to 7.4 with HCl. Add distilled H2O to 1 liter. Use at 1X (containing 0.1% Tween-20).
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